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VOLUME 12 , ISSUE 4 ( July-August, 2021 ) > List of Articles

ORIGINAL RESEARCH

Detection of Filifactor alocis and Dialister pneumosintes in Biofilms of Smokers and Nonsmokers with Chronic Periodontitis: A PCR Study

Shwetha Eshwarappa, Chethana K Chidambar, Shrinidhi M Shankar

Keywords : Biofilm, Dialister pneumosintes, Filifactor alocis, Polymerase chain reaction, Smokers

Citation Information : Eshwarappa S, Chidambar CK, Shankar SM. Detection of Filifactor alocis and Dialister pneumosintes in Biofilms of Smokers and Nonsmokers with Chronic Periodontitis: A PCR Study. World J Dent 2021; 12 (4):301-305.

DOI: 10.5005/jp-journals-10015-1846

License: CC BY-NC 4.0

Published Online: 15-07-2021

Copyright Statement:  Copyright © 2021; Jaypee Brothers Medical Publishers (P) Ltd.


Abstract

Aim and objective: Detection of Filifactor alocis and Dialister pneumosintes in smokers and nonsmokers with chronic generalized periodontitis. Materials and methods: Out of 60 patients diagnosed with chronic generalized periodontitis, 30 current smokers and 30 nonsmokers were recruited for the study. Clinical parameters such as gingival bleeding index, plaque index, and probing pocket depth were recorded. Subgingival plaque samples were collected and subjected to a multiplex polymerase chain reaction (PCR) assay. Results: Collected plaque samples of nonsmokers with chronic periodontitis patients showed a slight increase in numbers of F. alocis and D. pneumosintes species when compared with smokers, although statistically not significant. Conclusion: The distribution of F. alocis and D. pneumosintes which are considered as the masked pathogens of the periodontal disease was not significantly different in nonsmokers and smokers with chronic periodontitis. Clinical significance: These species which were rarely studied can be considered as markers for periodontal disease, as they denote increased periodontal destruction.


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