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VOLUME 12 , ISSUE 4 ( July-August, 2021 ) > List of Articles


Detection of Filifactor alocis and Dialister pneumosintes in Biofilms of Smokers and Nonsmokers with Chronic Periodontitis: A PCR Study

Shwetha Eshwarappa, Chethana K Chidambar, Shrinidhi M Shankar

Keywords : Biofilm, Dialister pneumosintes, Filifactor alocis, Polymerase chain reaction, Smokers

Citation Information : Eshwarappa S, Chidambar CK, Shankar SM. Detection of Filifactor alocis and Dialister pneumosintes in Biofilms of Smokers and Nonsmokers with Chronic Periodontitis: A PCR Study. World J Dent 2021; 12 (4):301-305.

DOI: 10.5005/jp-journals-10015-1846

License: CC BY-NC 4.0

Published Online: 15-07-2021

Copyright Statement:  Copyright © 2021; The Author(s).


Aim and objective: Detection of Filifactor alocis and Dialister pneumosintes in smokers and nonsmokers with chronic generalized periodontitis. Materials and methods: Out of 60 patients diagnosed with chronic generalized periodontitis, 30 current smokers and 30 nonsmokers were recruited for the study. Clinical parameters such as gingival bleeding index, plaque index, and probing pocket depth were recorded. Subgingival plaque samples were collected and subjected to a multiplex polymerase chain reaction (PCR) assay. Results: Collected plaque samples of nonsmokers with chronic periodontitis patients showed a slight increase in numbers of F. alocis and D. pneumosintes species when compared with smokers, although statistically not significant. Conclusion: The distribution of F. alocis and D. pneumosintes which are considered as the masked pathogens of the periodontal disease was not significantly different in nonsmokers and smokers with chronic periodontitis. Clinical significance: These species which were rarely studied can be considered as markers for periodontal disease, as they denote increased periodontal destruction.

  1. Ferraro CTL, Gornic C, Barbosa AS, et al. Detection of Dialister pneumosintes in the subgingival biofilm of subjects with periodontal disease. Anerobe 2007;13(5-6):244–248. DOI: 10.1016/j.anaerobe.2007.09.002.
  2. Shaikh HF, Patil SH, Pangam TS, et al. Polymicrobial synergy and dysbiosis: an overview. J Indian Soc Periodontol 2018;22(2):101. DOI: 10.4103/jisp.jisp_385_17.
  3. Berezow AP, Darveau RP. Microbial shift and periodontitis. Periodontol 2000 2011;55(1):36–47. DOI: 10.1111/j.1600-0757.2010.00350.x.
  4. Schlafer S, Riep B, Griffen AL, et al. Filifactor alocis-involvement in periodontal biofilms. BMC Microbiol 2010;10(1):66. DOI: 10.1186/1471-2180-10-66.
  5. Mason MR, Preshaw PM, Nagaraja HN, et al. The subgingival microbiome of clinically healthy current and never smokers. ISME J 2015;9(1):268. DOI: 10.1038/ismej.2014.114.
  6. Newman M, Takei HH, Klokkevold PR, et al. Carranza's clinical periodontology. 10th ed., St. Louis, Mo: Elsevier Saunders; 2006. 14: Smoking and Periodontal Disease pp. 251–258.
  7. Armitage GC. Development of a classification system for periodontal diseases and conditions. Ann Periodontol 1999;4(1):1–6. DOI: 10.1902/annals.1999.4.1.1.
  8. Silness J, Loe H. Periodontal disease in pregnancy. II. Correlation between oral hygiene and periodontal condition. Acta Odontol Scand 1964;22(1):121–135. DOI: 10.3109/00016356408993968.
  9. Loe H. The gingival index, the plaque index and the retention index systems. J Periodontol 1967;38(6 Part II):610–616. DOI: 10.1902/jop.1967.38.6_part2.610.
  10. Siqueira Jr JF, Rocas IN. Simultaneous detection of Dialister pneumosintes and Filifactor alocis in endodontic infections by 16S rDNA-directed multiplex PCR. J Endod 2004;30(12):851–854. DOI: 10.1097/01.DON.0000132300.13023.5D.
  11. Vanpelt E, Belkum VAV, Hays JP. Principles and technical aspects of PCR amplification. Springer; 2008. p. 34.
  12. Karasneh JA, Al Habashneh RA, Marzouka NA, et al. Effect of cigarette smoking on subgingival bacteria in healthy subjects and patients with chronic periodontitis. BMC Oral Health 2017;17(1):64. DOI: 10.1186/s12903-017-0359-4.
  13. Qiu F, Liang CL, Liu H, et al. Impacts of cigarette smoking on immune responsiveness: Up and down or upside down? Oncotarget 2017;8(1):268. DOI: 10.18632/oncotarget.13613.
  14. Aruni AW, Roy F, Fletcher HM. Filifactor alocis has virulence attributes that can enhance its persistence under oxidative stress conditions and mediate invasion of epithelial cells by Porphyromonas gingivalis. Infect Immun 2011;79(10):3872–3886. DOI: 10.1128/IAI. 05631-11.
  15. Jansson L, Ehnevid H, Blomlöf L, et al. Endodontic pathogens in periodontal disease augmentation. J Clin Periodontol 1995;22(8):598–602. DOI: 10.1111/j.1600-051x.1995.tb00811.x.
  16. Gomes SC, Piccinin FB, Oppermann RV, et al. Periodontal status in smokers and never-smokers: Clinical findings and real-time polymerase chain reaction quantification of putative periodontal pathogens. J Periodontol 2006;77(9):1483–1490. DOI: 10.1902/jop.2006.060026.
  17. Wang Q, Wright CJ, Dingming H, et al. Oral community interactions of Filifactor alocis in vitro. PLoS ONE 2013;8(10):e76271. DOI: 10.1371/journal.pone.0076271.
  18. Huang R, Li M, Ye M, et al. Effects of nicotine on Streptococcus gordonii growth, biofilm formation, and cell aggregation. Appl Environ Microbiol 2014;80(23):7212–7218. DOI: 10.1128/AEM. 02395-14.
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