World Journal of Dentistry

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VOLUME 12 , ISSUE 4 ( July-August, 2021 ) > List of Articles

ORIGINAL RESEARCH

Comparative Evaluation of Antifungal Activity of Green Coffee and Green Tea Extract against Candida albicans: An In Vitro Study

Indu Mathur, Sethuraman Shruthi, Kavya Gandrakota, Krishnavilasom Jayakumari Nisha

Keywords : Antifungal agents, Candida albicans, Chronic periodontitis, Coffee, Tea

Citation Information : Mathur I, Shruthi S, Gandrakota K, Nisha KJ. Comparative Evaluation of Antifungal Activity of Green Coffee and Green Tea Extract against Candida albicans: An In Vitro Study. World J Dent 2021; 12 (4):265-270.

DOI: 10.5005/jp-journals-10015-1839

License: CC BY-NC 4.0

Published Online: 15-07-2021

Copyright Statement:  Copyright © 2021; The Author(s).


Abstract

Aim and objective: To compare the antifungal efficacy of green tea and green coffee extracts against Candida albicans. Materials and methods: Green tea and green coffee were obtained from the authorized dealers. The extracts were prepared and antifungal efficacy of the extract was carried out on C. albicans MTCC 227 using zone of inhibition and minimum fungicidal concentration (MFC) methods. Results: The study comprises two groups, green tea extract and green coffee extract. The zone of inhibition was done for both the groups at 10, 25, 50, 100, 150, and 200 mg/mL. Green coffee showed a higher zone of inhibition at all the concentrations gradually from 50 C as compared to green tea. Green coffee showed a 50% reduction of C. albicans at 160 mg/mL and a 90% reduction at 200 mg/mL. On the other hand, green tea showed a 50% reduction at 200 mg/mL. Conclusion: In the present study, green coffee was shown to have lower inhibitory concentration and higher zone of inhibition when compared to green tea suggesting that the antifungal efficacy of green coffee was better than green tea. Clinical significance: Recently, many studies have shown the prominent role of Candida species especially C. albicans in the pathology of periodontal disease. Till now, there have been no studies that have included antifungal agents as adjuncts to periodontal therapy targeting C. albicans. Moreover, commercially available antifungal drugs come with many side effects. Green coffee and green tea being natural agents with antimicrobial, antioxidant, antifungal, antiviral, anti-inflammatory, and anticancer potential can definitely prove to be a viable option to the general population. Hence, opening the venue to formulate drugs in the form of mouthwashes, gels, and local drug delivery agents which can be used as adjuncts to periodontal therapy.


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  1. Olsen I, Lambris JD, Hajishengallis G. Porphyromonas gingivalis disturbs host-commensal homeostasis by changing complement function. J Oral Microbiol 2017;9(1):1340085. DOI: 10.1080/20002297.2017.1340085.
  2. Settem RP, Hassan AT, Honma K, et al. Fusobacterium nucleatum and Tannerella forsythia induce synergistic alveolar bone loss in a mouse periodontitis model. Infect Immun 2012;80(7):2436–2443. DOI: 10.1128/IAI.06276-11.
  3. Suresh Unniachan A, Krishnavilasom Jayakumari N, Sethuraman S. Association between Candida species and periodontal disease: a systematic review. Curr Med Mycol 2020;6(2):63–68. DOI: 10.18502/CMM.6.2.3420.
  4. Ali DN, Pardis H, Parisa B, et al. Antifungal effects of common mouthwashes on Candida strains colonized in the oral cavities of liver transplant recipients in south Iran. Hepat Mon 2016;16(1):31245. DOI: 10.1186/s12906-016-1332-5.
  5. Hirasawa M, Takada K. Multiple effects of green tea catechin on the antifungal activity of antimycotics against Candida albicans. J Antimicrob Chemother 2004;53(2):225–229. DOI: 10.1093/jac/dkh046.
  6. Zahra N, Mohammad Y. Antifungal activity of Caffeine in combination with fluconazole against Candida albicans. Infect Epidemiol Med 2016;2(2):18–21. DOI: 10.18869/modares.iem.2.2.18.
  7. Bharath N, Sowmya NK, Mehta DS. Determination of antibacterial activity of green coffee bean extract on periodontogenic bacteria like Porphyromonas gingivalis, Prevotella intermedia, Fusobacterium nucleatum and Aggregatibacter actinomycetemcomitans: an vitro study. Contemp Clin Dent 2015;6(2):166–169. DOI: 10.4103/0976-237X.156036.
  8. Axelrod M, Berkowitz S, Dhir R, et al., The inhibitory effects of green tea (CAMELLIA SINENSIS) on the growth and proliferation of oral bacteria. 2009.
  9. Magaldi S, Mata-Essayag S, Hartung de Capriles C, et al. Well diffusion for antifungal susceptibility testing. Int J Infect Dis 2004;8(1):39–45. DOI: 10.1016/j.ijid.2003.03.002.
  10. Rakatama SA, Pramono A, Yulianti R. The antifungal inhibitory concentration effectiveness test from ethanol seed arabica coffee (Coffea arabica) extract against the growth of Candida albicans patient isolate with In Vitro method. J Phys Conf ser 2018;970:012023. DOI: 10.1088/1742-6596/970/1/012023.
  11. Sachin M, Jiji J, Poonam S, et al. To evaluate the antimicrobial efficacy of green coffee bean extract on periopathogens. Natl J Integr Res Med 2016;7(5):56–59.
  12. Venkateswara B, Sirisha K, Chava VK. Green tea extract for periodontal health. J Indian Soc Periodontol 2011;15(1):18–22. DOI: 10.4103/0972-124X.82258.
  13. Nobuyuki K, Makoto M, Ryuichiro K, et al. Antifungal activity of components of essential oils, agricultural and biological chemistry. 1981;45(4):945–952.
  14. Yi TL, Shah M, Raulji D, et al. Comparative evaluation of antimicrobial efficacy of coffee extract and 0.2% chlorhexidine mouthwash on the periodontal pathogens Porphyromonas gingivalis, Prevotella intermedia, Fusobacterium nucleatum and Aggregatibacter actinomycetemcomitans: an in vitro study. Adv Hum Biol 2016;6(2):99–103. DOI: 10.4103/2321-8568.190316.
  15. Farhad Mollashahi N, Bokaeian M, Farhad Mollashahi L, et al. Antifungal efficacy of green tea extract against Candida albicans biofilm on tooth substrate. J Dent (Tehran) 2015;12(8):592–598.
  16. Halit A, Ercisli S, Yesil DZ, et al. Antifungal activity of green tea leaves (Camellia sinensis L.) sampled in different harvest time. Phcog Mag 2009;5(20):437–440.
  17. Samadi FM, Suhail S, Sonam M, et al. Antifungal efficacy of herbs. J Oral Biol Craniofac Res 2019;9(1):28–32. DOI: 10.1016/j.jobcr.2018. 06.002.
  18. Fazly Bazzaz BS, Sarabandi S, Khameneh B, et al. Effect of catechins, green tea extract and methylxanthines in combination with gentamicin against Staphylococcus aureus and Pseudomonas aeruginosa: - combination therapy against resistant bacteria. J Pharmacopuncture 2016;19(4):312–318. DOI: 10.3831/KPI.2016.19.032.
  19. Araghizadeh A, Kohanteb J, Fani MM. Inhibitory activity of green tea (Camellia sinensis) extract on some clinically isolated cariogenic and periodontopathic bacteria. Med Princ Pract 2013;22(4):368–372. DOI: 10.1159/000348299.
  20. Barroso H, Ramalhete R, Domingues A, et al. Inhibitory activity of a green and black tea blend on Streptococcus mutans. J Oral Microbiol 2018;10(1):1481322. DOI: 10.1080/20002297.2018.1481322.
  21. Arora DS, Ohlan D. In vitro studies on antifungal activity of tea (Camellia sinensis) and coffee (Coffea arabica) against wood-rotting fungi. J Basic Microbiol 1997;37(3):159–165. DOI: 10.1002/jobm.3620370302.
  22. Stavric B, Klassen R, Watkinson B, et al. Variability in caffeine consumption from coffee and tea: possible significance for epidemiological studies. Food Chem Toxicol 1988;26(2):111–118. DOI: 10.1016/0278-6915(88)90107-x.
  23. Salehi B, Lopez-Jornet P, Pons-Fuster López E, et al. Plant-derived bioactives in oral mucosal lesions: a key emphasis to curcumin, lycopene, chamomile, aloe vera, green tea and coffee properties. Biomolecules 2019;9(3):106. DOI: 10.3390/biom9030106.
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