Aim and objective: The aim and objective of this study was to estimate mast cell (MC) count in histopathological dysplasia grades in oral leukoplakia (OL) patients and in histopathological grades in oral squamous cell carcinoma (OSCC) patients and thereby determining their possible role in these lesions pathogenesis.
Materials and methods: Forty histopathologically confirmed cases of OL and OSCC were taken. Sections of 4 microns thickness were taken from each paraffin block and stained with 1% standard toluidine blue stain for demonstrating MCs. Counting of MCs was done by 3 observers using a 10× eyepiece fitted with 1 cm2 graticule and 40× objectives in 10 different fields in each slide.
Results: It was found that the MC count was higher in OSCC than OL and control group and the difference was found to be statistically significant. (One way Analysis of Variance test) Also, the MC count increased from mild to severe dysplasia in OL patients, as well as from well to poorly differentiated grades in OSCC patients (p < 0.01).
Conclusion: This study indicates that MC count was increased from mild to moderate to severe dysplasia of OL patients and also from well differentiated (WD) to moderately differentiated (MD) to poorly differentiated (PD) grades of OSCC patients.
Clinical significance: MC count was increased in higher grades of epithelial dysplasia in OL patients and higher grades in OSCC patients.
Spoorthi BR, Vidya GS. Mast cell count analysis in oral inflammatory lesions, potentially malignant disorders and oral squamous cell carcinoma. Int J Sci Res Publicat 2013;3(12):1–4.
Weller CL, Collington SJ, Williams T, et al. Mast cells in health and disease. Clin Sci 2011;120(11):473–484. DOI: 10.1042/CS20100459.
Okayama Y, Kawakami T. Development, migration, and survival of mast cells. Immunol Res 2006;34(2):97–115. DOI: 10.1385/IR:34:2:97.
Rakesh S, Janardhanan M, Vinodkumar RB, et al. Analysis of mast cell counts in oral leukoplakia. Oral Maxillofac Pathol 2012;3(1):181–185.
Teófilo CR, Ferreira Junior AEC, Batista AC, et al. Mast cells and blood vessels profile in oral carcinogenesis: an immunohistochemistry study. Asian Pac J Cancer Prev 2020;21(4):1097–1102. DOI: 10.31557/APJCP.2020.21.4.1097.
Michailidou EZ, Markopoulos AK, Antoniades DZ. Mast cells and angiogenesis in oral malignant and premalignant lesions. Open Dentis J 2008;2:126–132.
Ankle MR, kale AD, Nayak R. Mast cells are increased in leukoplakia, oral submucous fibrosis, oral lichen planus and oral submucous fibrosis. J Oral Maxillofac Pathol 2007;11(1):18–22. DOI: 10.4103/0973-029X.33959.
Singh S, Gupta V, Vij R, et al. Evaluation of mast cells in oral premalignant and malignant lesions: a histochemical study. Natl J Maxillofac Surg 2018;9(2):184–190. DOI: 10.4103/njms.NJMS_49_17.
Asboe-Hansen G. Mast cells in health and disease. Bull N Y Acad Med 1968;44(8):1048–1056.
Basha S, Monsur Hassan NM, Akhter R, et al. Emerging evidence of mast cell involvement in oral squamous cell carcinoma. Biomed J Sci Tech Res 2019;16(2):11954–11959.
Ramshridhar S, Narasimhan M. Immunohistochemical evaluation of mast cells in leukoplakia and oral squamous cell carcinoma. J Clin Diagnos Res 2016;10(8):100–103.
Iamaroon A, Pongsiriwet S, Jittidecharaks S, et al. Increase of mast cells and tumor angiogenesis in oral squamous cell carcinoma. J Oral Pathol Med 2003;32(4):195–199. DOI: 10.1034/j.1600-0714.2003.00128.x.
Biviji AT. Mast cells in normal and leukoplakia of buccal mucosa. J Indian Dent Assoc 1973;45:189–191.
Jandinski JJ, Sonis S, Doku HC. The incidence of mast cells in selected oral lesions. Oral Surg Oral Med Oral Pathol 1972;34(2):245–248. DOI: 10.1016/0030-4220(72)90415-X.
Zhao ZZ, Savage NW, Sugarman PB, et al. Mast cell/T cell interactions in oral lichen planus. J Oral Pathol Med 2002;31(4):189–195. DOI: 10.1034/j.1600-0714.2002.310401.x.
Fattahi S, Hosseini SV, Emamverdizadeh P, et al. The compare number of mast cells in biopsy specimens of patients with erosive & non-erosive lichen planus. Int J Curr Res Aca Rew 2014;2(9):124–134.
Ashkavandi ZJ, Mashhadi-Abbas F, Sargolzaie S, et al. Evaluation of CD31 expression and mast cell count in dysplastic lesions and squamous cell carcinoma of the oral cavity. Iran Red Crescent Med J 2010;12(3):272–276.
Cheema VS, Ramesh V, Balamurali PD. The relevance of mast cells in oral squamous cell carcinoma. J Clin Diagnos Res 2012;6(10):1803–1807. DOI: 10.7860/JCDR/2012/4503.2616.
Flier JS, Underhill LH, Galli SJ. New concepts about mast cells. N Engl J Med 1993;328(4):257–265. DOI: 10.1056/NEJM199301283280408.
Walsh LJ. Mast cells and oral inflammation. Crit Rev Oral Biol Med 2003;14(3):188–198. DOI: 10.1177/154411130301400304.