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VOLUME 11 , ISSUE 6 ( November-December, 2020 ) > List of Articles


Evaluation of Antimicrobial Efficacy of Commercially Available Herbal Products as Irrigants and Medicaments in Primary Endodontic Infections: In Vivo Study

Goldy Rathee, Monika Tandan

Keywords : Antimicrobial, Chlorhexidine gluconate, Herbal medicine, Neem juice extract, Primary endodontic infection, Tulsi juice extract

Citation Information : Rathee G, Tandan M. Evaluation of Antimicrobial Efficacy of Commercially Available Herbal Products as Irrigants and Medicaments in Primary Endodontic Infections: In Vivo Study. World J Dent 2020; 11 (6):488-493.

DOI: 10.5005/jp-journals-10015-1787

License: CC BY-NC 4.0

Published Online: 03-04-2021

Copyright Statement:  Copyright © 2020; The Author(s).


Aim: The purpose of this study was to comparatively evaluate the antimicrobial efficacy of two commercially available herbal products as root canal irrigants and medicaments—an in vivo study. Materials and methods: Thirty patients of age group 15–50 years with single-rooted teeth were selected. After access opening and working length (WL) determination, the pretreatment sample (S1) was obtained using a sterile paper point dipped in transport media. Biomechanical preparation (BMP) was done with master apical file size #40 and step-back up to size #60. The patient was randomly divided into three groups and irrigation was done. Group I—2% chlorhexidine gluconate (CHX), group II—neem juice extract, and group III—tulsi juice extract. Post-instrumentation sample (S2) was taken in the same manner. Canals were dried and three solutions were dispensed as intracanal medicaments. A double seal of Cavit was placed. After 7 days, post-medicament sample (S3) was collected in the same manner as S1. Microbiological samples (S1, S2, S3) were incubated and then plated on brain heart infusion agar and the colonies were counted. Collected data were statistically analyzed using repeated measures of analysis of variance (ANOVA). Results: The results of this study demonstrated that a statistically significant reduction was seen among all the groups during the intragroup comparison. The reduction in S3 from S1 was found to be significantly higher than that found in S2 from S1, which was further significantly higher than that found in S3 from S2. In intergroup comparison, the difference in colony counts reached the level of statistical significance in S2 from S1 and in S3 from S1 in all the three experimental solutions. Among these stages, a significant reduction was seen in group I and group II, group I and group III. Conclusion: Therefore, within the limitations of this study, it can be concluded that herbal products have shown significant antimicrobial activity in primary endodontic infections when compared to 2% CHX. Hence, they can be recommended as endodontic irrigants and medicaments. Clinical significance: Herbal products can be recommended as endodontic irrigants and medicaments.

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