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VOLUME 11 , ISSUE 6 ( November-December, 2020 ) > List of Articles


Low Frequency of Human Papillomavirus and Epstein-Barr Virus DNA in Ameloblastoma of Thai Patients

Dusit Bumalee, Puangwan Lapthanasupkul, Eakapong Tamboon, Anchisa Aittiwarapoj, Boworn Klongnoi, Nakarin Kitkumthorn

Keywords : Ameloblastoma, DNA, Epstein-Barr virus, Human papillomavirus, Thai

Citation Information : Bumalee D, Lapthanasupkul P, Tamboon E, Aittiwarapoj A, Klongnoi B, Kitkumthorn N. Low Frequency of Human Papillomavirus and Epstein-Barr Virus DNA in Ameloblastoma of Thai Patients. World J Dent 2020; 11 (6):446-450.

DOI: 10.5005/jp-journals-10015-1771

License: CC BY-NC 4.0

Published Online: 03-04-2021

Copyright Statement:  Copyright © 2020; The Author(s).


Aim: This preliminary study aimed to evaluate the frequency of human papillomavirus (HPV) and Epstein-Barr virus (EBV) DNA in solid/multicystic ameloblastoma (AM). Materials and methods: A total of 20 fresh-frozen AM tissue was investigated for HPV and EBV DNA using polymerase chain reaction (PCR) at L1 of HPV and LMP1 of EBV. Results: It was found that 2 out of 20 cases (10%) were positive for HPV PCR, whereas 3 out of 20 cases (15%) were positive for EBV PCR. Importantly, a mix of HPV and EBV DNA was observed in one case. Conclusion: Our study presented a low frequency of HPV and EBV DNA in AM. Further studies with different cohorts and larger sample sizes are necessary for a better understanding of the frequency of both viruses in AM. Clinical significance: HPV and EBV may not be involved in ameloblastogenesis.

  1. Yanatatsaneejit P, Boonsuwan T, Mutirangura A, et al. XRCC1 gene polymorphisms and risk of ameloblastoma. Archs Oral Biol 2013;58(6):583–589. DOI: 10.1016/j.archoralbio.2012.10.016.
  2. Worawongvasu R, Tiensuwan M. Odontogenic tumors in Thailand: a study of 590 Thai patients. J Oral Maxillofac Surg Med Pathol 2015;27(4):567–576. DOI: 10.1016/j.ajoms.2014.11.004.
  3. Effiom O, Ogundana O, Akinshipo A, et al. Ameloblastoma: current etiopathological concepts and management. Oral Dis 2018;24(3):307–316. DOI: 10.1111/odi.12646.
  4. Kahn MA. Ameloblastoma in young persons: a clinicopathologic analysis and etiologic investigation. Oral Surg Oral Med Oral Pathol 1989;67(6):706–715. DOI: 10.1016/0030-4220(89)90013-3.
  5. Correnti M, Rossi M, Avila M, et al. Human papillomavirus in ameloblastoma. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2010;110(3):e20–e24. DOI: 10.1016/j.tripleo.2010.04.030.
  6. Niedobitek G, Meru N, Delecluse HJ. Epstein-Barr virus infection and human malignancies. Int J Exp Pathol 2001;82(3):149–170. DOI: 10.1111/j.1365-2613.2001.iep190.x.
  7. zur Hausen H. Papillomaviruses in the causation of human cancers—a brief historical account. Virology 2009;384(2):260–265. DOI: 10.1016/j.virol.2008.11.046.
  8. Pandhi D, Sonthalia S. Human papilloma virus vaccines: current scenario. Indian J Sex Transm Dis AIDS 2011;32:75–85.
  9. Bhattarakosol P, Lertworapreecha M, Kitkumthorn N, et al. Survey of human papillomavirus infection in cervical intraepithelial neoplasia in Thai women. J Med Assoc Thai 2002;85:S360–S365.
  10. Kitkumthorn N, Yanatatsanajit P, Kiatpongsan S, et al. Cyclin A1 promoter hypermethylation in human papillomavirus-associated cervical cancer. BMC Cancer 2006;6(1):55. DOI: 10.1186/1471-2407-6-55.
  11. Badrawy H, Abd-Elmagid A, Hosam M, et al. Detection of EBV and HPV in oral squamous cell carcinoma and ameloblastoma: real time polymerase chain reaction study. JCTI 2014;1(1):7–18. DOI: 10.9734/JCTI/2014/15874.
  12. Shannon-Lowe C, Rickinson A. The global landscape of EBV-associated tumours. Front Oncol 2019;9:713. DOI: 10.3389/fonc.2019.00713.
  13. Yanatatsaneejit P, Mutirangura A, Kitkumthorn N. Human papillomavirus's physical state and cyclin A1 promoter methylation in cervical cancer. Int J Gynecol Cancer 2011;21(5):902–906. DOI: 10.1097/IGC.0b013e3182158683.
  14. Morales-Sánchez A, Fuentes-Pananá E. Human viruses and cancer. Viruses 2014;6(10):4047–4079. DOI: 10.3390/v6104047.
  15. El-Naggar A, Chan J, Takata T, et al. WHO classification of tumours. Pathology and genetics of head and neck tumours. Lyon: IARC Press; 2017.
  16. Sambrook J, Russell DW. Purification of nucleic acids by extraction with phenol: chloroform. Cold Spring Harbor Protocols 2006;2006(1):pdb.prot4455.
  17. de Roda Husman A-M, Walboomers JMM, van den Brule AJC, et al. The use of general primers GP5 and GP6 elongated at their 3′ ends with adjacent highly conserved sequences improves human papillomavirus detection by PCR. J Gen Virol 1995;76(4):1057–1062. DOI: 10.1099/0022-1317-76-4-1057.
  18. Wanvimonsuk S, Thitiwanichpiwong P, Keelawat S, et al. Distribution of the Epstein-Barr virus in the normal stomach and gastric lesions in Thai population. J Med Virol 2019;91(3):444–449. DOI: 10.1002/jmv.25318.
  19. Fujita S, Shibata Y, Takahashi H, et al. Latent infection with Epstein-Barr virus in odontogenic disorders: comparison among ameloblastoma, dentigerous cyst and odontogenic keratocyst. Pathol Int 1997;47(7):449–453. DOI: 10.1111/j.1440-1827.1997.tb04523.x.
  20. Jang HS, Cho JO, Yoon CY, et al. Demonstration of Epstein–Barr virus in odontogenic and nonodontogenic tumors by the polymerase chain reaction (PCR). J Oral Pathol Med 2001;30(10):603–610. DOI: 10.1034/j.1600-0714.2001.301005.x.
  21. Khalele BA. The anecdote of viral etiopathogenia in ameloblastoma and odontogenic keratocyst: why don't we let it go? J Oral Biol Craniofac Res 2017;7(2):101–105. DOI: 10.1016/j.jobcr.2017.04.002.
  22. Kahn MA. Demonstration of human papillomavirus DNA in a peripheral ameloblastoma by in situ hybridization. Hum Pathol 1992;23(2):188–191. DOI: 10.1016/0046-8177(92)90242-U.
  23. Heerden WFP, Rensburg EJ, Raubenheimer EJ, et al. Detection of human papillomavirus DNA in an ameloblastoma using the in situ hybridization technique. J Oral Pathol Med 1993;22(3):109–112. DOI: 10.1111/j.1600-0714.1993.tb01039.x.
  24. Sand L, Jalouli J, Larsson P-A, et al. Presence of human papilloma viruses in intraosseous ameloblastoma. J Oral Maxillofac Surg 2000;58(10):1129–1134. DOI: 10.1053/joms.2000.9573.
  25. Namin AK, Azad TM, Eslami B, et al. A study of the relationship between ameloblastoma and human papilloma virus. J Oral Maxillofac Surg 2003;61(4):467–470. DOI: 10.1053/joms.2003. 50011.
  26. Migaldi M, Pecorari M, Rossi G, et al. Does HPV play a role in the etiopathogenesis of ameloblastoma? an immunohistochemical, in situ hybridization and polymerase chain reaction study of 18 cases using laser capture microdissection. Mod Pathol 2005;18(2):283. DOI: 10.1038/modpathol.3800241.
  27. Mokhtari-Azad T, Moradi A, Khodayari-Nemin A, et al. Detection of human papillomavirus DNA in intraosseus ameloblastoma. Intl J Virol 2006;2(1):1–6. DOI: 10.3923/ijv.2006.1.6.
  28. Verduin L, Bishop J, Mills SE. Gnathic and peripheral ameloblastomas lack human papillomavirus DNA. Ann Diagn Pathol 2015;19(5):306–309. DOI: 10.1016/j.anndiagpath.2015.06.006.
  29. Singh T, Angel C, Tabrizi S, et al. Ameloblastoma, human papillomavirus, and p16-is there an association. Arch Otolaryngol Rhinol 2016;2:20–24. DOI: 10.17352/2455-1759.000016.
  30. Zare Mahmoud Abadi R, Saghafi S, Mohajertehran F, et al. Detection of human papillomavirus (HPV) in ameloblastoma using the polymerase chain reaction (PCR). J Mash Dent Sch 2019;42:298–306.
  31. Shi Y, Peng S-L, Yang L-F, et al. Co-infection of Epstein-Barr virus and human papillomavirus in human tumorigenesis. Chin J Cancer 2016;35(1):16. DOI: 10.1186/s40880-016-0079-1.
  32. Stavrou S, Ross SR. APOBEC3 proteins in viral immunity. J Immunol 2015;195(10):4565–4570. DOI: 10.4049/jimmunol.1501504.
  33. Fanourakis G, Tosios K, Papanikolaou N, et al. Evidence for APOBEC3B mRNA and protein expression in oral squamous cell carcinomas. Exp Mol Pathol 2016;101(3):314–319. DOI: 10.1016/j.yexmp.2016. 11.001.
  34. Kikuchi K, Inoue H, Miyazaki Y, et al. Epstein–Barr virus (EBV)-associated epithelial and non-epithelial lesions of the oral cavity. Jpn Dent Sci Rev 2017;53(3):95–109. DOI: 10.1016/j.jdsr.2017. 01.002.
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