World Journal of Dentistry

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VOLUME 11 , ISSUE 4 ( July-August, 2020 ) > List of Articles

ORIGINAL RESEARCH

Evaluation of Periodontal Ligament Cell Viability in Honey as a Storage Media at Different Time Intervals: An In Vitro Study

Priyal P Sheth, Nikita V Lolayekar, Amitha M Hegde, Veena A Shetty

Citation Information : Sheth PP, Lolayekar NV, Hegde AM, Shetty VA. Evaluation of Periodontal Ligament Cell Viability in Honey as a Storage Media at Different Time Intervals: An In Vitro Study. World J Dent 2020; 11 (4):310-315.

DOI: 10.5005/jp-journals-10015-1756

License: CC BY-NC 4.0

Published Online: 07-10-2020

Copyright Statement:  Copyright © 2020; The Author(s).


Abstract

Aim: To evaluate the viability of human periodontal ligament (PDL) cells in honey when used as a storage media at different time intervals. Materials and methods: Fifty freshly extracted human premolars were divided into four experimental groups: group I: stored in honey immediately after extraction; group II: stored in honey after 30 minutes extraoral dry time; group III: in Hank's balanced salt solution (HBSS) immediately (positive control); and group IV: bench-dried for 8 hours with no media (negative control). Groups I, II and III were further divided into three subgroups, to test viability at different time intervals of 3, 6, and 24 hours. The PDL tissue derived was subjected to the trypan blue dye exclusion test. The number of viable cells was estimated with a hemocytometer and the data were statistically analyzed, and the level of significance was kept at p < 0.05. Results: Groups I and III showed no statistically significant difference in percentage of viable PDL cells after 3 hours (p = 0.339) and 6 hours (p = 0.142), and group II had a statistically lower percentage of viable cells compared to groups I and III (p < 0.001). After 24 hours, group III had highest percentage of viable cells. Group IV had a consistently lower percentage of viable cells. Conclusion: Within the limitations of this study, it appears that honey may be as efficient as HBSS for storage of avulsed teeth up to 6 hours. Clinical significance: Commercial honey meets most requirements of an ideal storage media. Being more readily available, inexpensive, and having several therapeutic properties can make it a popular storage media for short duration of storage.


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