World Journal of Dentistry

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VOLUME 11 , ISSUE 3 ( May-June, 2020 ) > List of Articles

ORIGINAL RESEARCH

Evaluation of Pathological Microbes in Root Canals with Pulp Necrosis

Atul Anand Bajoria, Ahmed Ali Alfawzan, Vardharajula Venkata Ramaiah, Mohammed Ali Habibullah, Sabahat Ullah Tareen

Keywords : Bacteria, Gram positive, Periapical

Citation Information : Bajoria AA, Alfawzan AA, Ramaiah VV, Habibullah MA, Tareen SU. Evaluation of Pathological Microbes in Root Canals with Pulp Necrosis. World J Dent 2020; 11 (3):211-214.

DOI: 10.5005/jp-journals-10015-1723

License: CC BY-NC 4.0

Published Online: 27-07-2020

Copyright Statement:  Copyright © 2020; The Author(s).


Abstract

Aim: The present study was conducted to evaluate the type of bacteria present in necrotic root canals of permanent teeth. Materials and methods: All 60 participants with infected root canals were made to use 10 mL of mouthwash containing 0.12% chlorhexidine. Access to pulp chamber was established, and the sterile absorbent paper cones were inserted into the canal for 20 seconds. The contaminated paper cones were inoculated in a brain–heart infusion (BHI) agar culture medium and incubated in an oven for 48 hours at 37°C. Results were analyzed statistically with SPSS version 20.0 using Chi-square test and analysis of variance (ANOVA). Results: In root canals with periapical lesions, gram-positive bacilli was present in 50 cases, gram-negative in 48 and yeast cells in 28; while in root canals without periapical lesions, gram-positive bacilli was present in 8. In 16 root canals of chronic apical periodontitis cases, gram-positive bacteria was present in 100%, gram-negative bacteria in 100%, and yeast cells in 20% cases. In 12 cases of periapical granuloma, gram-positive bacteria was present in 98%, gram-negative bacteria in 100%, and yeast cells in 40% cases. In 10 cases of chronic abscess with fistula, gram-positive bacteria was present in 86.2. In six cases of phoenix abscess, gram-positive bacteria was present in 100% and gram-negative bacteria in 100% cases. Conclusion: There is predominantly gram-positive and -negative bacteria as well as the presence of yeast cells in periapical lesions. Clinical significance: Knowledge about the bacterial species in root canal infection helps in its management and in selecting antibiotic.


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