World Journal of Dentistry

Register      Login

VOLUME 11 , ISSUE 3 ( May-June, 2020 ) > List of Articles

ORIGINAL RESEARCH

Evaluation of Pathological Microbes in Root Canals with Pulp Necrosis

Atul Anand Bajoria, Ahmed Ali Alfawzan, Vardharajula Venkata Ramaiah, Mohammed Ali Habibullah, Sabahat Ullah Tareen

Keywords : Bacteria, Gram positive, Periapical

Citation Information : Bajoria AA, Alfawzan AA, Ramaiah VV, Habibullah MA, Tareen SU. Evaluation of Pathological Microbes in Root Canals with Pulp Necrosis. World J Dent 2020; 11 (3):211-214.

DOI: 10.5005/jp-journals-10015-1723

License: CC BY-NC 4.0

Published Online: 30-09-2020

Copyright Statement:  Copyright © 2020; The Author(s).


Abstract

Aim: The present study was conducted to evaluate the type of bacteria present in necrotic root canals of permanent teeth. Materials and methods: All 60 participants with infected root canals were made to use 10 mL of mouthwash containing 0.12% chlorhexidine. Access to pulp chamber was established, and the sterile absorbent paper cones were inserted into the canal for 20 seconds. The contaminated paper cones were inoculated in a brain–heart infusion (BHI) agar culture medium and incubated in an oven for 48 hours at 37°C. Results were analyzed statistically with SPSS version 20.0 using Chi-square test and analysis of variance (ANOVA). Results: In root canals with periapical lesions, gram-positive bacilli was present in 50 cases, gram-negative in 48 and yeast cells in 28; while in root canals without periapical lesions, gram-positive bacilli was present in 8. In 16 root canals of chronic apical periodontitis cases, gram-positive bacteria was present in 100%, gram-negative bacteria in 100%, and yeast cells in 20% cases. In 12 cases of periapical granuloma, gram-positive bacteria was present in 98%, gram-negative bacteria in 100%, and yeast cells in 40% cases. In 10 cases of chronic abscess with fistula, gram-positive bacteria was present in 86.2. In six cases of phoenix abscess, gram-positive bacteria was present in 100% and gram-negative bacteria in 100% cases. Conclusion: There is predominantly gram-positive and -negative bacteria as well as the presence of yeast cells in periapical lesions. Clinical significance: Knowledge about the bacterial species in root canal infection helps in its management and in selecting antibiotic.


HTML PDF Share
  1. Reddy GA, Sridevi E, Sai Sankar AJ, et al. Endodontic treatment of chronically infected primary teeth using triple antibiotic paste: An in vivo study. J Conserv Dent 2017;20(6):405–410. DOI: 10.4103/JCD.JCD_161_17.
  2. Sakko M, Tjäderhane L, Rautemaa-Richardson R. Microbiology of root canal infections. Prim Dent J 2016;5(2):84–89. DOI: 10.1308/205016816819304231.
  3. de Mendonça Cavalcante A, Soares NM, Santos IC, et al. Assessment of microbiota in root canals with pulp necrosis by means of Gram test. Afr J Microbiol Res 2018;12(22):508–511. DOI: 10.5897/AJMR2017.8762.
  4. Gomes BP, Berber VB, Kokaras AS, et al. Microbiomes of endodontic-periodontal lesions before and after chemomechanical preparation. J Endod 2015;41(12):1975–1984. DOI: 10.1016/j.joen.2015.08.022.
  5. Tzanetakis GN, Azcarate-Peril MA, Zachaki S, et al. Comparison of bacterial community composition of primary and persistent endodontic infections using pyrosequencing. J Endod 2015;41(8):1226–1233. DOI: 10.1016/j.joen.2015.03.010.
  6. Narayanan LL, Vaishnavi C. Endodontic microbiology. J Conserv Dent 2010;13(4):233–239. DOI: 10.4103/0972-0707.73386.
  7. Dudeja PG, Dudeja KK, Srivastva D, et al. Microorganisms in periradicular tissues: Do they exist? A perennial controversy. J Oral Maxillofac Pathol 2015;19(3):356–363. DOI: 10.4103/0973-029X.174612.
  8. Filho FJ. Microbial analysis of canals of root-filled teeth with periapical lesions using polymerase chain reaction. J Endod 2008;34(5):537–540. DOI: 10.1016/j.joen.2008.01.016.
  9. Pourhajibagher M, Ghorbanzadeh R, Bahador A. Culture dependent approaches to explore the prevalence of root canal pathogens from endodontic infections. Braz Oral Res 2017;31:1–7. DOI: 10.1590/1807-3107bor-2017.vol31.0108.
  10. Nóbrega LM, Montagner F, Ribeiro AC, et al. Bacterial diversity of symptomatic primary endodontic infection by clonal analysis. Braz Oral Res 2016;30(1):e103. DOI: 10.1590/1807-3107BOR-2016.vol30.0103.
  11. Gomes BPFA. Etiologic role of root canal infection in apical periodontitis and its relationship with clinical symptomatology. Braz Oral Res 2018;32(Suppl 1):e69. DOI: 10.1590/1807-3107bor-2018.vol32.0069.
  12. Vengerfeldt V, Špilka K, Saag M, et al. Highly diverse microbiota in dental root canals in cases of apical periodontitis (data of illumina sequencing). J Endod 2014;40(11):1778–1783. DOI: 10.1016/j.joen.2014.06.017.
  13. Lana MA, Ribeiro-Sobrinho AP, Stehling R, et al. Microorganisms Isolated From Root Canals Presenting Necrotic Pulp and Their Drug Susceptibility in Vitro. Oral Microbiol Immunol 2001;16(2):100–105. DOI: 10.1034/j.1399-302x.2001.016002100.x.
  14. Peciuliene V, Maneliene R, Balcikonyte E, et al. Microorganisms in root canal infections: a review. Stomatologija 2008;10(1): 4–9.
  15. Jhajharia K. Microbiology of endodontic diseases: A review article. Int J Appl Dent Sci 2019;5(1):227–230.
PDF Share
PDF Share

© Jaypee Brothers Medical Publishers (P) LTD.