World Journal of Dentistry

Register      Login

VOLUME 11 , ISSUE 3 ( May-June, 2020 ) > List of Articles

ORIGINAL RESEARCH

Effectiveness of Star Fruit Leaf Extract on the Growth of Streptococcus Sanguinis: An In Vitro Study

Minasari Nasution, Yolanda Simatupang, Dennis Dennis

Keywords : Minimum inhibition concentration, Star fruit leaf extract, Streptococcus sanguinis,Minimum bactericidal concentration

Citation Information : Nasution M, Simatupang Y, Dennis D. Effectiveness of Star Fruit Leaf Extract on the Growth of Streptococcus Sanguinis: An In Vitro Study. World J Dent 2020; 11 (3):196-200.

DOI: 10.5005/jp-journals-10015-1731

License: CC BY-NC 4.0

Published Online: 27-07-2020

Copyright Statement:  Copyright © 2020; Jaypee Brothers Medical Publishers (P) Ltd.


Abstract

Aim: The study was aimed to examine the effectiveness level of minimum inhibition concentration (MIC) and minimum bactericidal concentration (MBC) of the star fruit leaf extract on the growth of Streptococcus sanguinis (S. sanguinis) bacteria at concentration 60, 40, 20, 10, and 5%. Materials and methods: This study was laboratory experimental research with the posttest-only control group design. The effectiveness test of the star fruit leaf extract on the growth of S. sanguinis was performed with dilution method. The leaf extract of star fruit was made by the maceration technique from each concentration, suspension of S. sanguinis bacteria was added, repetition done four times, and then observation was conducted. The data analysis used was the one-way ANOVA test. Results: The result showed that MIC concentration of the extract on the growth of S. sanguinis was 5% and MBC concentration was 10%. Conclusion: The star fruit leaf extract has antimicrobial efficacy against S. sanguinis. Clinical significance: The star fruit leaf extract has the potential capacity to prevent and cure dental caries, recurrent aphthous stomatitis (RAS), and periodontal disease.


PDF Share
  1. Republic of Indonesia ministry of health's health research and development agency. Basic Health Research (RISKESDAS). Jakarta 2013. pp. 118–119.
  2. Liantari DS. Effect of star fruit leaf extract for streptococcus mutans growth. J Majority 2014;3(7):27–33.
  3. Sriherfyna FH, Zubaidah. Physical-chemical characteristics and antibacterial activity of star fruit leaf extract (Averrhoa bilimbi L.). J Food Agroind 2016;4(1):400–409.
  4. Sari M, Suryani C, Effect of star fruit extract (Averrhoa bilimbi L) in inhibiting the growth of the fungus Candida albicans in vitro In: Department of Faculty of Mathematics and Natural Sciences Universitas Negeri Medan. Conference proceedings of National Seminar in Biology and learning, 2014: pp. 325–32.
  5. Yulianingtyas A, Kusmartono B. Optimization of solvent volume and maceration time of star fruit leaf extract (Averrhoa bilimbi L). J Chem Sci 2016;10(2):58–64.
  6. Zheng W, Tan TK, Paterson IC, et al. Streptobase an oral streptococcus mitis group genomic resource and analysis platform. PLoS. ONE. 2016;1l(5):1–19. DOI: 10.1371/journal.pone.0151908.
  7. Suwandi T. Development of antibacterial potential of hibiscus sabdariffa L. (Rosela) petals against Streptococcus sanguinis inducing gingivitis towards standardized herbal medicines. Dissertation. Jakarta: Universitas Indonesia; 2012. pp. 19–22.
  8. Ge X, Kitten T, Chen Z, et al. Identification of Streptococcus sanguinis genes required for biofilm formation and examination of their role in endocarditis virulence. Infect lmmun 2008;76(6):2551–2559. DOI: 10.1128/IAI.00338-08.
  9. Anggraini N, Saputra O. Efficacy of star fruit (Averrhoa bilimbi L.) on the healing of acne vulgaris. J Majority 2016;5(1):76–80.
  10. Brooks GF, Butel JS, Ornston LN, et al. Medical microbiology. Translation by Maulany NRF Jakarta: Medical Book Publisher; 1996. p. 219.
  11. Philip K, Teoh WY, Muniandy S, et al. Pathogenic bacteria predominate in oral cavity of Malaysian subjects. J Biolog Sci 2009;9(5):438–444. DOI: 10.3923/jbs.2009.438.444.
  12. Nurdiana JusriM. Management of major recurrent aphthous stomatitis with secondary infections. J Dentofas 2011;10(1):44–46.
  13. Taliningrum KK, Kholifa M, Rochmanita N. The difference in the various concentrations of 70% ethanol extract of star fruit leaf (Averrhoa bilimbi L.) as mouthwash to inhibit the growth of sStreptococcus anguis bacteria. Universit Muhammad Surak 2015. 2–11.
  14. Morita C, Sumioka R, Nakata M, et al. Cell wall-anchored nuclease of Streptococcus sanguinis contributes to escape from neutrophil extracellular trap-mediated bactericidal activity. J P One 2014;9(8):1–10. DOI: 10.1371/journal.pone.0103125.
  15. Marsh PD, Martin MV, Lewis MAD, et al. Oral microbiology. 5th ed., China: Churchill Livingstone ELSEVIER; 2009. p. 33.
  16. Alhassan AM, Ahmed QU. Averrhoa bilimbi linn.: a review of its ethnomedicinal uses, phytochemistry, and pharmacology. J. Pharm. Bioallied Sci. 2016;8(4):265–271. DOI: 10.4103/0975-7406.199342.
  17. Kumar KA, Gousia SK, Anupama. A review on phytochemical constituents and biological assays of Averrhoa bilimbi L. Int J Pharma Pharmaceut Sci Res 2013;3(4):136–139.
PDF Share

© Jaypee Brothers Medical Publishers (P) LTD.