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VOLUME 11 , ISSUE 3 ( May-June, 2020 ) > List of Articles

ORIGINAL RESEARCH

Assessment of Salivary Hemoglobin as Noninvasive Biomarker of Chronic Periodontitis in Type II Diabetics

Sunila B Sangappa, SubbaRao V Madhunapantula, Bettahalli S Avinash, Kenkere M Srinath, Basavagowda Madhu, Shivamurthy Ravindra, Appattira U Chinnappa

Keywords : Diabetes mellitus, Periodontitis, Salivary hemoglobin, Tooth loss

Citation Information : Sangappa SB, Madhunapantula SV, Avinash BS, Srinath KM, Madhu B, Ravindra S, Chinnappa AU. Assessment of Salivary Hemoglobin as Noninvasive Biomarker of Chronic Periodontitis in Type II Diabetics. World J Dent 2020; 11 (3):189-195.

DOI: 10.5005/jp-journals-10015-1725

License: CC BY-NC 4.0

Published Online: 27-07-2020

Copyright Statement:  Copyright © 2020; The Author(s).


Abstract

Aim: The present study aimed at determining the association between salivary hemoglobin (SH) and chronic periodontitis in type II diabetic subjects and check whether SH can function as a noninvasive biomarker to assess the level of chronic periodontitis. Materials and methods: This cross-sectional comparative study included 40 subjects aged between 40 and 65 years with at least 20 teeth remaining. The diabetic status was confirmed with HbA1c levels and categorized into group I: healthy controls with HbA1c < 5.6% and no periodontitis with bleeding on probing (BOP) at ≤10% of sites, <3% of sites with probing pocket depth (PPD) ≥4 mm, and no sites with clinical attachment level (CAL) ≥ 2 mm and group II: type II diabetes mellitus (T2DM) cases with HbA1c > 5.7% and periodontitis with BOP at >10% of sites, with CAL ≥ 2 mm and with >5% of sites with PPD ≥ 4 mm. Unstimulated fasting whole saliva was collected from each participant and the salivary hemoglobin level analyzed using a colorimetric assay kit. Both groups were compared using the t-test and multiple linear regression model analysis. Relationship between different variables were compared using the Karl Pearson's correlation coefficient. Statistical significance was set at 5% level (p < 0.05). Results: A significant difference was observed between the salivary hemoglobin level (t −3.7710, p < 0.001), PPD (t −13.9023, p < 0.001), and CAL (t −9.3759, p < 0.001) between healthy controls and type II diabetics with T2DM subjects exhibiting much higher value compared to healthy controls. Conclusion: In conclusion, data from this study demonstrated that type II diabetic adults have high prevalence of chronic periodontitis and exhibit elevated salivary hemoglobin indicating a valuable noninvasive screening method for detecting periodontitis.


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