World Journal of Dentistry

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VOLUME 11 , ISSUE 1 ( January-February, 2020 ) > List of Articles

ORIGINAL RESEARCH

Modification to the Centrifugation Protocol Increases the Competence of Platelet-rich Fibrin in Controlled Diabetic Patients? A Light Microscopic Study

Jai Abooj, Alluri Siddhartha Varma, Girish Suragimath, Sameer A Zope, Apurva Pisal

Keywords : Controlled diabetic, Periodontitis, Platelet-rich fibrin, Regeneration

Citation Information : Abooj J, Varma AS, Suragimath G, Zope SA, Pisal A. Modification to the Centrifugation Protocol Increases the Competence of Platelet-rich Fibrin in Controlled Diabetic Patients? A Light Microscopic Study. World J Dent 2020; 11 (1):65-68.

DOI: 10.5005/jp-journals-10015-1693

License: CC BY-NC 4.0

Published Online: 01-02-2020

Copyright Statement:  Copyright © 2020; The Author(s).


Abstract

Aim: This study aims to evaluate and compare the structural characteristics of platelet-rich fibrin (PRF) prepared by two different protocols in nondiabetic and controlled diabetic patients. Materials and methods: Ten milliliters of blood sample were drawn from 30 participants [nondiabetic (15), controlled diabetic (15)]. Five milliliters of blood were transferred to two dry vacutainers. For each patient two PRF clots were obtained using the standard protocol [leukocyte-rich platelet-rich fibrin (L-PRF)] and the low-speed concept [advanced platelet-rich fibrin (A-PRF)]. These were processed for light microscopy analysis to estimate the width of the fibrin meshwork. Results: In controlled diabetic patients, A-PRF showed significantly more dense fibrin network compared to those of L-PRF. The difference between L-PRF and A-PRF in nondiabetic patients was not statistically significant. Conclusion: Modification done to the standard centrifugation protocol, by increasing time and reducing speed, favors an increase in the dense fibrin network of A-PRF in diabetic patients. Clinical significance: Modified centrifugation protocol proves to be favorable in controlled diabetic patients for periodontal regeneration.


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