Immunohistochemical Expression of Fascin in Oral Epithelial Dysplasia and Oral Squamous Cell Carcinoma
Shilpa C Natesan, Bindhu P Ramakrishnan, Rekha Krishnapillai, Priya Thomas
Fascin, Head and neck cancer, Immunohistochemistry, Oral cancer, Oral epithelial dysplasia, Oral leukoplakia, Oral squamous cell carcinoma
Citation Information :
Natesan SC, Ramakrishnan BP, Krishnapillai R, Thomas P. Immunohistochemical Expression of Fascin in Oral Epithelial Dysplasia and Oral Squamous Cell Carcinoma. World J Dent 2019; 10 (5):340-345.
Aims: The present study aimed to evaluate and compare the expression of fascin in normal oral mucosa (NOM) and oral epithelial dysplasia (OED) and to estimate the expression in different grades of oral squamous cell carcinoma (OSCC).
Materials and methods: The study involves a total of 55 cases including 30 cases of OSCC, 15 cases of OED, and 10 NOM tissues. All the sections were subjected to fascin immunostaining. Epithelial dysplasia were scored by layer-wise immunohistochemical staining, while for carcinoma staining intensity and number of cells stained were assessed. The scores were analyzed using one-way ANOVA test and Tukey's post hoc test.
Results: Our results showed that fascin expression in NOM was very low or restricted to the basal layers of the epithelium. In case of OED and OSCC, fascin immunostaining was highly elevated. Also, it was seen increasing with increasing grades of dysplasia (p = 0.002) and decrease in differentiation of OSCC.
Conclusion: A steady up-regulation of fascin expression is noted in OED and squamous cell carcinoma. These findings suggest that fascin plays an important role from early stages of carcinogenesis to invasive carcinoma.
Clinical significance: Fascin can be used as a reliable biomarker for diagnostic and prognostic implications of OED and OSCC, respectively.
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