World Journal of Dentistry

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VOLUME 10 , ISSUE 5 ( September-October, 2019 ) > List of Articles

ORIGINAL RESEARCH

Determination of Microbiological Salivary Status in Children with Upper Respiratory Tract Disorders

Valë H Hoxha, Agim Begzati, Vlorë H Cakolli

Keywords : Enlarged tonsils, Lactobacilli, Middle ear infection, Mutans streptococci, Oral health

Citation Information : Hoxha VH, Begzati A, Cakolli VH. Determination of Microbiological Salivary Status in Children with Upper Respiratory Tract Disorders. World J Dent 2019; 10 (5):327-334.

DOI: 10.5005/jp-journals-10015-1664

License: CC BY-NC 4.0

Published Online: 01-10-2019

Copyright Statement:  Copyright © 2019; The Author(s).


Abstract

Aim: The purpose of this paper is to investigate the prevalence of dental caries and the types of streptococci mutant and Lactobacillus in the saliva of children with middle ear infection (MEI), enlarged tonsils (ET), and healthy children. Materials and methods: A sample of 93 subjects (57 males and 36 females; mean age: 8.1 ± 1 years, aged between 5 years and 15 years old) were assessed: 35 patients, with MEI, and 26 with ET, formed the study group, while 32 healthy subjects formed the control group. The participants were interviewed regarding demographic data, dietary, and oral hygiene habits. Their dental caries and oral hygiene status were clinically determined, assessing diagnostic criteria for decayed, missing, and filled teeth for primary (dmft) and permanent (DMFT) dentition, oral hygiene index according to Greene–Vermillion, and the gingival index (GI) according to Silness–Löe. Stimulated salivary flow rate, and salivary mutans streptococci (MS) and lactobacilli (LB) were also determined. Results: The groups were similar with respect to gender, using fluoride paste, visits to dentist, family income (p > 0.05), whereas there was a difference in age, educational level, method of feeding, use of pacifier, sweet eating habits, toothbrushing, saliva secretion, and rate of oral breathing (p < 0.001) between intervention and healthy group. However, higher salivary MS and LB levels were observed in MEI, and ET patients compared to controls (p < 0.05). Conclusion: Our results demonstrated that patients with ET and MEI had more initial caries, gingival inflammation, dental plaque, a lower stimulated salivary secretion rate, and an increase in the level of salivary MS and LB than healthy individuals. Clinical significance: Results from our study will help not only the pediatric dentistry specialists but also the other medical professionals like pediatricians, ENT specialists, and nurses to increase their awareness regarding the importance of the overall dental health in children with an ear infection or tonsillitis.


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  1. Gomes-Filho IS, Passos JS, da Cruz SS. Respiratory disease and the role of oral bacteria. J Oral Microbiol 2010;2:5811. DOI: 10.3402/jom.v2i0.5811.
  2. Paju S, Bernstein JM, Haase EM, et al. Molecular analysis of bacterial flora associated with chronically inflamed maxillary sinuses. J Med Microbiol 2003;52(Pt 7):591–597. DOI: 10.1099/jmm.0.05062-0.
  3. Zhou Y, Jiang S, Li KY, et al. Association between oral health and upper respiratory tract infection among children. Int Dent J 2018;68(2):122–128. DOI: 10.1111/idj.12335.
  4. Duncan B, Ey J, Holberg CJ, et al. Exclusive breast feeding for at least 4 months protects against otitis media. Pediatrics 1993;91(5):867–872.
  5. Eryaman E, Oter Ilhan B, Erdinc A. Any relation between poor oral hygiene and tonsillar hypertrophy? KBB-Forum 2013;12(1):1–4.
  6. Singh S, Vijayakumar N, Priyadarshini HR, et al. Prevalence of early childhood caries among 3–5 year old pre-schoolers in schools of Marathahalli, Bangalore. Dent Res J (Isfahan) 2012;9:710–714.
  7. Edwardsson S, Koch G, Obrink M. Strep. sanguis, Strep. mutans and Strep. salivarius in saliva. Prevalence and relation to caries increment and prophylactic measures. Odontol Revy 1972;23(3):279–296.
  8. Köhler B, Andréen I. Mutans streptococci and caries prevalence in children after early maternal caries prevention: a follow-up at 19 years of age. Caries Res 2012;46(5):474–480. DOI: 10.1159/000339665.
  9. Marsh PD. Microbiology of dental plaque biofilms and their role in oral health and caries. Dent Clin North Am 2010;54(3):441–454. DOI: 10.1016/j.cden.2010.03.002.
  10. Seki M, Karakama F, Terajima T, et al. Evaluation of mutans streptococci in plaque and saliva: correlation with caries development in preschool children. J Dent 2003;31(5):283–290. DOI: 10.1016/S0300-5712(03)00033-2.
  11. Flanagan D. Inspissated oral secretions and a review of their clinical, biological, and physiological significance. Spec Care Dentist 2012;32(3):99–104. DOI: 10.1111/j.1754-4505.2012.00241.x.
  12. Ersin NK, Gülen F, Eronat N, et al. Oral and dental manifestations of young asthmatics related to medication, severity and duration of condition. Pediatr Int 2006;48(6):549–554. DOI: 10.1111/j.1442-200X.2006.02281.x.
  13. WHO: Oral Health Country, Area Profile Programme Oral Hygiene Indices, (on web) http:/www.whocollob.od.mah.se/expl/ohiintrod.html.
  14. WHO: World Oral Health County, Area Profile World Health Organization Oral Health Surveys. Basic Methods, 4th ed., Geneva: World Health Organization; 1997.
  15. Löe H. The gingival index, the plaque index and the retention index systems. J of Periodontology 1967;38(6 Part II):610–616. DOI: 10.1902/jop.1967.38.6_part2.610.
  16. Simplified Oral Hygiene Index|OHI-S—Oral Health Database, Malmö University; 1964.
  17. Mummolo S, Nota A, Caruso S, et al. Salivary markers and microbial flora in mouth breathing late adolescents. BioMed Res Int 2018;2018:8687608. DOI: 10.1155/2018/8687608.
  18. Bakhshaee M, Ashtiani SJ, Hossainzadeh M, et al. Allergic rhinitis and dental caries in preschool children. Dent Res J 2017;14(6):376–381. DOI: 10.4103/1735-3327.218560.
  19. Wongkamhaeng K, Poachanukoon O, Koontongkaew S. Dental caries, cariogenic microorganisms and salivary properties of allergic rhinitis children. Int J Pediatr Otorhinolaryngol 2014;78(5):860–865. DOI: 10.1016/j.ijporl.2014.03.001.
  20. Widmer RP. Oral health of children with respiratory diseases. Paediatr Respir Rev 2010;11(4):226–232. DOI: 10.1016/j.prrv.2010.07.006.
  21. Niklander S, Veas L, Barrera C, et al. Risk factors, hyposalivation and impact of xerostomia on oral health-related quality of life. Brazilian Oral Research 2017;31:e14. DOI: 10.1590/1807-3107bor-2017.vol31.0014.
  22. Mehta A, Sequeira PS, Sahoo RC, et al. Is bronchial asthma a risk factor for gingival diseases? A control study. N Y State Dent J 2009;75(1):44–46.
  23. Kashyap N, Katlam T, Avinash A, et al. Middle ear infection in children and its association with dental caries. Medicine and Pharmacy Reports 2019;92(3):271.
  24. Eryaman E, Oter Ilhan B, Erdinc A. Poor oral hygiene and middle ear infections: any relationship? Indian J Otolaryngol Head Neck Surg 2013;65(2):173–176. DOI: 10.1007/s12070-012-0616-4.
  25. Nelson S, Nechvatal N, Weber J, et al. Dental caries and ear infections in preschool-aged children. Oral Health Prev Dent 2005;3(3):165–171.
  26. Mummolo S, Tieri M, Tecco S, et al. Clinical evaluation of salivary indices and levels of streptococcus mutans and Lactobacillus in patients treated with occlus-O-guide. Eur J Paediatr Dent 2014;15(4):367–370.
  27. Zhou Y, Yang JY, Zhi QH, et al. Factors associated with colonization of streptococcus mutans in 8–32-month-old children: a cohort study. Aust Dent J 2013;58(4):507–513. DOI: 10.1111/adj.12113.
  28. Köhler B, Andréen I, Jonsson B. The earlier the colonization by mutans streptococci, the higher the caries prevalence at 4 years of age. Oral Microbiol Immunol 1988;3(1):14–17. DOI: 10.1111/j.1399-302X.1988.tb00598.x.
  29. Kõll-Klais P, Mändar R, Leibur E, et al. High levels of salivary lactobacilli in Estonian school children. Eur J Paediatr Dent 2004;5(2):107–109.
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