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VOLUME 1 , ISSUE 1 ( April-June, 2010 ) > List of Articles


Grossing in Oral Pathology: General Principles and Guidelines

BR Premalatha, Roopa S Rao

Citation Information : Premalatha B, Rao RS. Grossing in Oral Pathology: General Principles and Guidelines. World J Dent 2010; 1 (1):35-41.

DOI: 10.5005/jp-journals-10015-1007

Published Online: 01-06-2010

Copyright Statement:  Copyright © 2010; The Author(s).


Grossing of surgical pathology specimens form the first and an important step in tissue processing, leading to diagnosis. It forms a connecting link between the patient and the pathologist. It involves a close coordination between the surgeon, pathologist and the histotechnologist. A correct grossing procedure helps in minimizing the processing errors while at the same time providing useful information about the specimen helping in the achievement of correct diagnosis. However, the importance of this step is often overlooked and neglected. The purpose of this article is to provide adequate insight about the grossing procedure of the pathologic specimens of head and neck region. Our effort is to present this article as a hands-on experience for the student population and the technicians.

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  1. Tools to facilitate and standardize grossing. Histo Logic 2003;36(1):17-21.
  2. Theory and practice of histological techniques. Churchill Livingstone Elsevier (6th ed). 2008;75-82.
  3. Rosai and Ackerman's Surgical Pathology (9th ed). Mosby Elsevier 2004;1:25-36.
  4. A new grossing knife with two parallel blades for preparing uniform thickness gross tissue sections. J Clin Pathol 2008;61(9):1069-70.
  5. Oral biopsies: Methods and applications. Br Dent J 2004;196:329-33.
  6. Manual of surgical pathology gross room procedures. University of Minnesota Press, Minneapolis. 1981;6.
  7. Avoiding artefacts in oral biopsies: The punch biopsy versus the incisional biopsy. Br J Oral Maxillofac Surg 1995;33(4):244-47.
  8. Biopsy and histopathologic diagnosis of oral premalignant and malignant lesions. J Can Dent Assoc Apr 2008;74(3):283-88.
  9. Artefacts in oral biopsy specimens. J Oral Maxillofacial Surg 1985;43:163-72.
  10. Biopsy technique: The pathological considerations. J Am Dent Assoc 1978;96:438-43.
  11. Common problems in biopsy procedures. J Oral Surg 1978;36:644-47.
  12. The oral mucosal punch biopsy: Indications and technique. J Am Dent Assoc 1990;121:145-49.
  13. Experience and knowledge of oral cancer and precancer among dentists in northwestern Spain. J Cancer Educ 1999;14:175-76.
  14. Histopathology for minor surgery. Histopathology 1992;20:455-56.
  15. Effect of fixatives and tissue processing on the content and integrity of nucleic acids. Am J Pathol 2002;161:1961-71.
  16. Oral biopsy technique. The pathologist's perspective. J Can Dent Assoc 1986;52:591-95.
  17. A technique for fixation of oral mucosal lesions. J Oral Maxillofac Surg 1992;50:1345
  18. Artefacts in oral incisional biopsies in general dental practice: A pathology audit, Oral Dis 2004;10:113-17.
  19. Available at Accessed on 2nd April, 2010.
  20. Grossing of bone and soft tissue (Common Specimens and Procedures). Humana press (2nd ed). 2009;125-28.
  21. Photography. In Westra WH, Hruban RH, Phelps TH, Isacson C (Eds): Surgical pathology dissection. An illustrated guide (2nd ed). New York. Springer-Verlag 2003;26-32.
  22. Gross specimen photography: A survey of lighting and background techniques. Med Biol Illustr 1975;25:159-66.
  23. Photography of medical specimens. Experiences from teaching cardiovascular pathology. Mayo Clin Proc 1988;63:42-57.
  24. Specimen Photography. In Hansell P (Ed): A Guide to Medical Photography. London, MTP press 1879;77-97.
  25. Recognition of quality photographs of gross specimens. Audiovisual teaching set. Iowa City, Iowa, Department of Pathology, University of Iowa, 1978.
  26. The color photogrphy of gross specimens. J Coll Am Pathologist 1984;3:155-61.
  27. Photomacrography, an introduction. Boston, Mass: Butterworth. 1987;97-133.
  28. Lymph node identification. Specimen radiography of tissue predominated by fat. Am J Clin Pathol 1977;68:511-12.
  29. Lymph node identification in carcinoma of the colon and rectum. Value of tissue specimen radiography. Acta Pathol Microbiol Scand (A) 1978;86:205-09.
  30. Lymph node identification by specimen radiography and xerography (letter). Am J Clin Pathol 1978;70:308-09.
  31. Microradiographic of bone, a new use for the versatile Faxitron(letter). Arch Pathol 1975;99:62.
  32. Dimenstein. Grossing biopsies: An introduction to general principles and techniques. Ann Diagn Pathol 2009;13:106-13.
  33. Ham's histology. JB Lippincott Company: Philadelphia (9th ed). 1987;3-23.
  34. Breast. In Westra WH, Hruban RH, Phelps TH, Isacson C (Eds). Surgical Pathology dissection. An illustrated guide (2nd ed). New York, Springer-Verlag 2003;132-41.
  35. Inking without a mess. J Clin Pathol 2008;61:783.
  36. Description and preliminary results of a novel cassette system (Tissue-tek Paraform Cassette System). HistoLogic 1999;31(2):28-30.
  37. A simple yet effective technique to improve laboratory safety for the grossing of large surgical specimens. Ann Diagn Pathol 2000;4(01):44-45.
  38. Bone grossing techniques: Helpful hints and procedures. Ann Diagn Pathol 2008;12(03):191-98.
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